Canine Endometrial Organoids as a Model to Investigate MSC - Derived Extracellular Vesicle Therapy for Enhanced Fertility in Bitches with Cystic Endometrial Hyperplasia
Category: Research Poster
Author(s): Charissa Ehresman
Presenter(s): Charissa Ehresman
Mentors(s): Fiona Hollinshead, Melinda Meyers
Organoids are a three-dimensional cell culture model that represent the in vivo environment and can be affected by hormones. This project utilizes organoids as an in vitro model of diseased canine endometrial tissue in order to examine the diseased tissue and test a potential treatment for the disease. The objective was to show the benefit of extracellular vesicles (EVs) at reducing inflammation, the leading symptom of cystic endometrial hyperplasia (CEH), which affects the uterine lining and pregnancy. EVs are nanoparticles secreted by cells that can be used both in cell culture and dosed in vivo. In this project, EVs are assessed for reducing inflammation in organoids developed from canine endometrial samples with CEH. Our results showed a statistically significant decrease in inflammatory markers when using PCR after EVs were added to CEH organoids. This finding can be further used in research regarding fertility of diseased subjects.